A Multi-layered Microfluidic Device for in Vitro Blood-brain Barrier Permeability Studies
نویسندگان
چکیده
BLOOD-BRAIN BARRIER PERMEABILITY STUDIES Ross Booth and Hanseup Kim Electrical and Computer Engineering, Bioengineering University of Utah, Salt Lake City, UT ABSTRACT The blood-brain barrier (BBB) blocks most compounds from entering the central nervous system (CNS), limiting the success rate of new treatments of CNS disease. Insight needed to overcome these limitations can be gained through innovation of more representative models of the BBB. We present the first multi-layered microfluidic device (MMD) for dynamic modeling of the BBB. Brain endothelial cell monolayers cultured in the device exhibited the following BBB properties: Tight junction formation as indicated by immunostaining, trans-endothelial electrical resistance (TEER) values of over 250Ωcm by day 3 of culture, and size-selective permeability of a range of test permeates.
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